Enter the details of the design you are interested in and a Fluoresentric Design Team member will contact you shortly to discuss your assay needs and provide you a no obligation custom project quotation. Design Only are always $175.00.

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   Real time PCR assay Design Form:                              

* required



Please complete as much of the form below as possible.
 
       
* SERVICE:   Consultation    Assay Design    Design and Confirmation  
       
* TARGET:    DNA RNA  
       
  NCBI Accession #:    
  (If not publicly available we need sequence data of ~200 bp, with reads in both directions. The sequence data should be submitted along with the design order. If numbering bases from sequence data, use the Forward primer sequence read as the reference sequence. The sequence data can be emailed to design@fluoresentric.com)  
       
* Project Description:
(ie: SNP analysis of codon A171G, or Detection of mRNA splice exon 1-2 versus exons 1-3.
Quantification of HCV viral load.)		  
   
       
       
       
* Real-Time PCR Instrument
Available for your use?		  
  Excitation Wavelengths for your instrument?  
  Detection Wavelengths for your instrument?  
       
  Probe Chemistry Preference?
TaqMan Roche Hyb Probes
SimpleProbe Molecular Beacon
Other - specify
 
       
       
       
  Would you like Fluoresentric to provide a Chemistry Recommendation?
Yes     No		  
       
* Do you have existing Thermal Cycling Protocol that this Design MUST work with?
Yes     No		  
  If YES, please provide EXACT pre-amplification, amplification, post-amplification parameters (including cycle #, target temperatures, temperature transition rates, and hold times).  
   
       
       
       
  Is there a specific buffer system that this Design MUST work with?
Yes     No		  
  If YES, please provide EXACT buffer system components and concentrations (include dNTP, Na+ salts, K+ salts, Mg++ salts, buffer type (Tris, HEPES, etc) and buffer pH).  
   
       
       
       
  If using a commercially available kit and the above information is unavailable to you, please provide Kit Manufacturer, Kit Name (Product #) and Lot # (most recent).  
   
       
       
       
  Is there a specific DNA Polymerase that MUST be used for this Design?
Yes     No		  
  If YES, please provide Enzyme Vendor Information (Vendor Name, Product #, Lot #):  
   
       
       
       
  For reverse transcription PCR do you have a specific Reverse Transcriptase that MUST be used?
Yes     No		  
  If YES, please provide Enzyme Vendor Information (Vendor Name, Product #, Lot #):  
   
     
     
 

* Institution
  Department
* Investigator
* Submission Date
* Investigator E-mail
* Confirmation E-mail
* Investigator Phone
  Best Time To Call

 
     
 
   

Phone 800.808.0490
Fax: 435.658.1408
info@fluoresentric.com



  
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