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Design and optimization of multiplex reactions
is more difficult than single-plex designs.
The intention to multiplex cannot be an afterthought in the design
process, but rather it must be a key consideration in the initial
assay design.
The experts at Fluoresentric have years of experience solving the
design issues faced in multiplex reactions.
Let us put that experience to work for you.
Call 1-800-808-0490 or E-mail us at info@fluoresentric.com
to learn how we can help you harness the power of multiplex PCR.
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A two-target multiplex reaction uses 50% less buffer, enzyme, and
vessels than
2 single-plex reactions. A three-target multiplex reaction uses
67% less buffer,
enzyme, and vessels than 3 single-plex reactions.
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Example Calculation: |
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2 x SinglePlex Assays |
1 x Duplex Assay |
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Primers & Probes |
$0.50 |
$0.50 |
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Buffer |
$2.00 |
$1.00 |
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Total $/ 2 Results |
$2.50 |
$1.50 |
A simple duplex can save $1.00/sample.
Can you really afford not to multiplex?
Reliable quantification data require internal reaction
controlsaction
controls
Internal controls allow PCR scientists to:
-establish the presence of reaction inhibitors
-compensate for anomylous setup variations
-normalize for sample preparation-control their PCR!
Real-time PCR instruments are expensive and instrument throughput
is a primary bottleneck in many laboratories.
Multiplexing helps alleviate this problem. By allowing a single
instrument to yield two to three times the data compared to using
the instrument solely to produce single-plex results.
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