Fluoresentric's designs and optimizes duplex, triplex, and quadruplex real-time PCR assays regularly.  We have the expertise to get your multiplex PCR assay up and running quickly.

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Save Time and Money with Multiplex PCR!

In the PCR world, "multiplexing" refers to detecting multiple targets in a single reaction.

The ability to multiplex is one of the most powerful aspects of real-time PCR, but most users are still performing single-plex reactions.

Why should you multiplex? Check out some reasons below

 



Design and optimization of multiplex reactions is more difficult than single-plex designs.

The intention to multiplex cannot be an afterthought in the design process, but rather it must be a key consideration in the initial assay design.


The experts at Fluoresentric have years of experience solving the design issues faced in multiplex reactions.


Let us put that experience to work for you.

Call 1-800-808-0490 or E-mail us at info@fluoresentric.com to learn how we can help you harness the power of multiplex PCR.

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Multiplex


A two-target multiplex reaction uses 50% less buffer, enzyme, and vessels than
2 single-plex reactions. A three-target multiplex reaction uses 67% less buffer,
enzyme, and vessels than 3 single-plex reactions.

Example Calculation:
  2 x SinglePlex Assays 1 x Duplex Assay
Primers & Probes $1.00 $1.00
Master Mix $2.00 $1.00
Total $/ 2 Results $3.00 $2.00

A simple duplex can save $1.00/sample.   Imagine the $ savings that a triplex, a 4-plex, or 5-plex can produce.

With our assay development guarantee, can you really afford not to multiplex?


Reliable quantification data require internal reaction controlsaction controls
Internal controls allow PCR scientists to:

 

-establish the presence of reaction inhibitors
-compensate for anomylous setup variations
-normalize for sample preparation-control their PCR!

Real-time PCR instruments are expensive and instrument throughput is a primary bottleneck in many laboratories.

Multiplex PCR alleviates bottlenecks.....Enabling a single instrument to yield two, three, four, or even FIVE times the data  of an instrument solely performing single-plex assays.