Take advantage of our expert design staff that develops the ready-to-use custom
real-time PCR assays. We provide custom R & D and Assay Validation for your
specific assay and laboratory needs.
This service is perfect for labs that
wish to outsource PCR and their R & D or wish to outsource PCR validation.
In over 800 designs our custom PCR assays have been greater than
99.7% Fluoresentric will design an assay to meet your specific
probe chemistry or instrument needs. If you are unsure of appropriate
detection chemistry our expert staff will recommend one. No confidential
information is required to get started.
Custom R & D and Assay Validation includes the following:
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PCR Validation and custom PCR service - Ensure sequences of primers designed to
meet your assay needs. We certify primer efficiency and ensure primer sequence
and primer specificity.
Certify the sequence(s) of the fluorescent probe(s) and check annealing
temperature optimization.
Guaranteed Performance - Oligonucleotide sequence(s) BLAST data to demonstrate design
specificity and ensure performance. (Our unique method of performing BLAST searches
eliminates undue sequence comparisons). We guarantee PCR performance.
Oligonucleotide alignment on target template.
Amplification protocol validations with recommendations and buffer R&D (thermal cycling
and buffer concentrations).
Custom R & D includes chemistry formulation and custom buffer formulation designed specifically for your
assay.
Custom PCR assays - Custom PCR, custom R&D, all specific for your assay.
ADDITIONAL INFORMATION:
Inquire about quality assured oligonucleotides for your Assay
Design.
Inquire about control templates.
Inquire about our assay optimization consultation services.