Development of a robust assay requires optimization of reaction conditions. With Fluoresentric’s FluorEssence package, we not only design your assay and confirm that the assay works, but also optimize the reaction conditions to meet your reaction sensitivity, and reaction condition requirements


FluorEssence Optimization Packages include the following:

  1. Fluoresentric consults with you via phone and/or e-mail to determine the type of assay that you require and the intended use of the assay.
  2. Fluoresentric then designs the probes and primers for a real time PCR (RTPCR) assay against a target sequence according to the needs specified by you.
  3. Using homology searches in appropriate genetic databases, Fluoresentric confirms the specificity of the probes and primer designs.
  4. Fluoresentric orders the appropriate probes and primers.
  5. Fluoresentric performs extensive quality assurance on the primers and probes.
  6. Fluoresentric optimizes the RTPCR reaction conditions (Quantification, Genotyping, Detection, etc.)
  7. Fluoresentric sends you ~200 reactions of quality assured (QA'd) probes and primers to conduct assays and specific written instructions to assist you in running your custom assay.
  8. Included are 2 hours of post-delivery phone consultation.

ADDITIONAL INFORMATION:


> Back to services page