Real Time PCR with probes allows accurate quantification of samples over six orders of magnitude. With the Radiance Quantification Package, Fluoresentric can do all the development work to get your assay up and running, so you can use your valuable time to collect data. In addition to a complete FluorEssence™ Optimization Package, Radiance Quantification Packages include the following::

  1. The development of an appropriately controlled assay (Choose between Internal Controls and External Controls).
  2. Determination of the limit of detection (LOD).
  3. Determination of the limit of quantification (LOQ).
  4. Construction of a appropriate standard curve.
  5. Establishment of the quantitative range of the assay.
  6. Up to 10 samples, to be quantified, are included in the development package.

TYPES OF QUANTIFICATION AVAILABLE:

Relative Quantification (Internal Control): View in our Store

  • For single target relative quantification, in addition to your custom probes and primers, Fluoresentric will provide pre-validated standard templates to act as an internal control for template concentration. concentration.
  • A two-target relative quantification assay will be designed to determine the ratio of one customer-specified target to a second specifi

Absolute Quantification (Internal Control): View in our Store

  • For absolute quantification, a synthetic standard for is also created and provided. created and proviAn absolute quantification includes the generation of a standard curve indicating copy number versus crossing threshold.

Relative Quantification (External Control): View in our Store

  • For single target relative quantification, in addition to your custom probes and primers, Fluoresentric will provide pre-validated standard templates to act as external controls for template concentration.ls for template concentration.
  • A two-target relative quantification assay will be designed to determine the ratio of one customer-specified reference target (housekeeping gene) to a second specified target of interest.

ADDITIONAL INFORMATION:

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